BIOL 200 Lecture Notes - Lecture 32: Selectable Marker, Thymidine, Pronucleus
Document Summary
To study gene functions, the best way is to get rid of them! When you get rid of them, there"d be some sort of stutter, error in cell function. Forward genetic analysis: used to understand a process" molecular origin. You insert a mutagen, create mutants, and figure out what genes are affected by this mutagenesis. Reverse genetic analysis: you know the gene, now you want to go back and figure out function, what it does in an organismal context like in mice; by eliminating gene function. Can carry out gene replacements via homologous recombinations (hr), in. Hr can be performed in the pluripotent stem cells (cid:871)es(cid:872)of mouse specific (unlike transgene which is random injection ), but expensive! You need to: a) know the sequence of the gene you want to target; b) come up with a gene replacement/targeting construct, this should be.
