MIMM 211 Lecture Notes - Lecture 2: Lazzaro Spallanzani, Louis Pasteur, Microbiological Culture
29 Aug 2016
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Lazzaro spallanzani: french academy of science sponsored competition to disprove spontaneous generation, louis pasteur - boiled meat broth in curved swan flask a. b. Disproved spontaneous gen: john tyndall proved that dust carried micro-org. Lucretius and fracastro suggested invisible organisms cause diseases. M. j. berkeley showed that irish potato blight was caused by fungus. Pasteur showed that protozoans caused another disease in silkworms. France then began producing silkworms from healthy moths: joseph lister- heat sterilized instruments and dipped surgical dressing in phenol. He was interested in pasteur"s work: robert koch- discovered anthrax causing bacteria. Koch"s postulates: see lecture slides/wiki, anywhere, etc. Difficult to work with liquid culture (mixed bacteria is a problem) o o o. Easier to attain pure culture as a colony progenated from one bacterium will be visible. Tried solidified liquid media (gelatin + meat broth) = semi-fail. Fannie eilshemius hesse (wife of one of koch"s assistants) o. Richard petri- (another one of koch"s assistants)- invented petri dish.
