PHGY 312 Lecture Notes - Lecture 8: Ouabain, Cardiac Glycoside, Nephron
24 May 2016
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Phgy 312-lecture 8: nacl transport ii and water transport: In the basolateral membrane of the renal tubule. Drives transepithelial transport, generates a na potential gradient. Na gradient is used by other transporters for 2nd active transport. 1 atp hydrolyzed to transport 3 na out and 2 k into the cell. Translocation involves conformational change from e1 to e2 (faces ec. Has phosphorylated intermediate, therefore it is a p -type atpase. Functional heterodimer of and b subunits: subunit is catalytic (does the actual transport), contains ouabain binding site o. B subunit is needed for assembly and export of pump to the membrane. 3 binding domains: a, p, and n binding. P is made of 2 parts, acts as phosphorylation domain. A is actuator domain, changes position and gates the pore. Contains multiple cell types, smooth transition from dt to cd. Voltage in the tubule becomes increasingly negative, in contrast to the positive.
