BIOCHEM 2EE3 Lecture Notes - Peripheral Membrane Protein, Atp Synthase, Intermembrane Space

• School: McMaster University
• Department: Biochemistry
• Course: BIOCHEM 2EE3
• Professor: Boris Zhorov

Document Summary

Learning objectives: enzyme complexes in the etc, chemiosmotic theory, structure of atp synthase, oxidation of 1 nadh = 10 h+ = 2. 5 atp/nadh molecule. Not strictly stoichiometric: 4h : 1 atp. It collects electrons from complexes i and ii and transfers them to complex iii. This complex passes electrons from the reduced coq to cytochrome c: the contex contains four redox centers, 2 cytochrome b, 1 cytochrome c, 1 fe-s cluster, cytochrome c is a peripheral membrane protein. It carries electrons in the intermembrane space from complex iii to complex iv. The obtained proton gradient is used as a driving force in the endergonic reaction of atp synthesis. Chemiosmotic theory: the free energy of electron transport is conserved by pumping. H+ from the matrix to the intermembrane space. The obtained electrochemical potential drives atp synthesis: proposed in 1961 by peter mitchel to explain the following observations: membrane.