BIOL 205 Lecture Notes - Centimorgan, Lysis, Rolling Circle Replication

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27 Sep 2013
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Ch 5 the genetics of bacteria and their viruses. Bacteria often carry extra dna elements called plasmids that are smaller than the main bacterial genome. Bacteria can be parasitized by viruses called bacteriophages which can have dna or rna as genetic material. Liquid culture of bacteria is spread onto an agar plate in the process of plating, this forms a clump of bacteria called a colony with members of a colony that have a single ancestor as a cell clone. Categories of mutants: wild type bacteria are prototrophic, meaning that they can grow on minimal medium, a substrate containing only inorganic salts. These types of mutants are genetic markers to keep track of genomes and cells in experiments. Discovered by lederberg and tatum who studied 2 strains of e. coli with different auxotrophic mutations. The strains were mixed together and plated on minimal medium where none of the auxotrophs could grow.

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