MICR 221 Lecture Notes - Lecture 4: Aeromonas Salmonicida, Campylobacter Fetus, Chelation

• School: Queen's University
• Department: Microbiology and Immunology
• Course: MICR 221
• Professor: Virginia K Walker

Document Summary

S layers forms a 2-d lattice on the surface like floor tiles single protein or glycoprotein. Types of s layer hexagonal, p6 symmetry: most archaea species with s layers have p6 tetragonal, p4 symmetry linear, p2 symmetry, rare cells always make their same pattern on the surface. S layer proteins acidic deficient in sulfur containing amino acids. 40-200 kda in size center to center spacing of 3-35 nm (always the same for a certain slayer: defined pore size. S layer isolation urea or guanidine hcl detergents chelating agents (edta) held together by non-covalent forces, makes it extremely easy to isolate. S layer: functions resistance to viruses, predatory bacteria (very common in nature) resistance to enzymes, antibodies, complement ion trap adhesion in some archaea, shape determination of cells. 1: campylobacter fetus: refractory to macrophage ingestion, many others i. e. chlamydia, treponema, clostridium. Commercial uses of s layers ultrafiltration membranes very tight molecular weight cutoff.