BMS 870 Lecture 5: W4L2 Neurotransmitter Systems
28 Oct 2017
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Document Summary
Nt systems can be defined by: the molecule, synthetic machinery, packaging, reuptake and degradation, and action. Acetylcholine - released by cholinergic (otti) neurons or noradrenergic (dale) neurons. When applied to a neuron, the chemical mimics postsynaptic cell response produced by release of nt from the presynaptic neuron. Immunohistochemistry - localize molecules to cells by using ab"s to recognize molecules involved. Raise ab in animal model -> wait for 2 weeks -> bleed it out from animal -> purify ab. Inject ab into animal model w/ adjuvant (physically irritating so that ab reacts to it) Run protein extract on sds polyacrylamide gel separated by size. Use dilution of blood to find out whether ab will bind to anything on your membrane. *animal shouldn"t already have ab against protein of interest. Secondary ab (anti-rabbit antibody) should be raised in a diff animal. Takes 2 weeks-1month to raise ab in animal.
