MBB 231 Lecture Notes - Lecture 12: Glycosylation, Amide, Transferase
9 Dec 2015
School
Department
Course
Professor
Document Summary
The endomembrane system is comprised up of the rough endoplasmic reticulum, smooth endoplasmic reticulum, Functions in disrupting the plasma membrane, releasing soluble proteins, and maintaining the integrity of. Organelles, and therefore gathering a sufficient amount of cells. This process occurs in a homogenizing buffer (usually cold and isotonic - 0. 25m sucrose), which will. Retain the integrity of the organelles of interest: centrifugation: There are 3 major types of centrifugation techniques: differential centrifugation. The separation of cell organelles of different size / density by their different rates of. Sedimentation rate: expressed as a sedimentation coefficient in terms of svedberg units (s). As the size of the molecule increases, the higher the sedimentation coefficient, and thus the more rapid the sedimentation: density gradient (rate - zonal) The sample is often isolated first by differential centrifugation. The sample (specific organelles) is layered on top of the solution that will separate the. Sample, which is generally a sucrose gradient: equilibrium density (buoyant density centrifugation)
