BIOCH200 Lecture Notes - Lecture 23: Skeletal Muscle, Cardiac Muscle, Conjugate Acid
17 Apr 2018
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Enzymes that catalyze irreversible reactions: (in biochemistry 200, regulated does not mean the substrate: hexokinase: first enzyme, pfk-1: most important enzyme, pyruvate kinase: last enzyme. Gapdh: not regulated, reversible, catalyzes the only oxidation state in glycolysis. Pfk1: allosterically (sigmoidal, homo-allosteric, more than 1 subunit) regulated by adp/amp and pep. Pep: build up means pyruvate kinase not working. Pyruvate kinase: enzyme (allostery), reciprocal (2 pathways, anabolic & catabolic) involved which are opposing) (when one is activated the other is inhibited e. g. gluconeogenesis and glycolysis) Energy put in is what makes it exogernic. Atp, isomerization, atp: fructose 1,6 bisphosphate which turns on pyruvate kinase and this produces 2 extra atp reaction. Absence of atp cause the sigmoidal graph to shift to the left/close to y axis and presence of. F-6-p + atp (substrate) > f-1,6-bp + adp + h+ (catalyzed by pfk1) Atp becomes an inhibitor when its concentration is very high. 2 binding sites: active site/substrate & allosteric site.
