MBIO 4612 Lecture Notes - Lecture 4: Rna-Seq, Transcriptome, Small Interfering Rna
9 Feb 2020
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Types: rrna catalytic component of ribosomes (80-85%) trna transfers amino acids to polypeptide chain at the ribosomal site of protein synthesis (15%) Mrna carries info about a protein sequence to the ribosome (5%) transcripts reflect which genes are being expressed studied via microarray. Mirna regulation of protein translation, gene expression. Dsrna functions vary sirna degrade mrnas and involved in protein expression snrna role in splicing and regulate splicing pathways. Transcriptome = all rna produced in a cell. Varies over time & with environmental conditions. Dna is physically attached to a chip (known genes at known locations) Transcripts are fluorescently labeled hybridized to chip & measured via fluorescence. Rna fragments converted to cdna linkers attached for identification. Short bits of sequence attached to glass slide act as primers for sequencing: sample cdna ligates to sequence on slide. Sequencing done with fluorescently labeled nt (adds 1 at a time to determine sequence like sanger)
