BIOL302 Lecture Notes - Lecture 4: Blood Film, Giemsa Stain, Eosinophil

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blueeagle732

13 Feb 2018

School

University of Waterloo

Department

Biology

Course

BIOL302

Professor

Mungo Marsden

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Document Summary

In this lab we will look at the circulatory system. While blood is often considered to be a distinct tissue it is properly classified as a connective tissue. Blood is composed of cells (red blood cells, leuckocytes, and platelets) as well as a variety of soluble matrix fibers and ground substance (plasma). In this lab you will identify the various cellular elements in a peripheral blood smear: erythrocytes (red blood cells = rbcs), leukocytes (white blood cells), and platelets. Try to identify the different types of leukocytes (lymphocytes, neutrophils, eosinophils, basophils, and monocytes). Learn the function of each and relate its function to the structures you can see. Erythrocytes are very common in tissue sections and provide a useful ruler for estimating the size of structures. Erythrocytes average about 8 m in diameter, and unlike leukocytes do not vary much in size. Note the variability in the size and nuclear shape of monocytes and lymphocytes.

Related Questions

I have a quiz for the cell structures and their functions. Please answer these questions.

1. CHEEK CELLS

Cheek cells are not the major producers of mucus in the mouth, they do produce a small amount of mucopolysaccharide. What inclusions should be especially numerous in the cytoplasm of these cells in order to make and excrete such a substance? What function does their flat shape provide?

2. BLOOD SMEAR (FROG & HUMAN)

How they differ? How is each adapted to the metabolic level of its owners? What is the function of these cells? What is the natural selection benefit of removing nuclei and mitochondria from the human red blood cells?

3. LEAF TISSUE (cross section)

Is there a cell membrane? A cell wall? Note the green pigment in the chloroplasts. Is green color found outside of chloroplasts? Why?

4. GIANT MULTIPOLAR NEURON

Look for the long fibers that support the processes. What cytoskeletal components are these composed of? Can you suggest a function for this characteristic?

5.SPERM (frog)

How do this structure and size relate to the function of a sperm?

6. ADIPOSE TISSUE

Look for cell boundaries and nuclei in the cytoplasmic areas. Can you suggest a function of these fat cells?

7.EPITHELIUM (columnar)

What is the internal structure of microvilli? What is their function?

jadeturtle730

1. Diseases are those abnormal conditionsin which the bodys defensive cells fail to make the self versus thenonself distinction correctly, and attack the body's owntissues.
A. Cancerous
B. Septic
C. Autoimmune
D. Nondiscrete

2. Which of the following determinesgenetic sex (male or female) in mammals?
A. the acrosome of the sperm
B. the temperature of the developing fetus
C. the SRY gene on the Y chromosome
D. the genetic makeup of the parents
E. the Y chromosome contained in the egg

3. Which of the following regardingantigens is true?
A. Antigens can only be recognized by their specificity.
B. An antigen is a molecule which promotes a general immuneresponse.
C. A large antigen is likely to have many different epitopes, eachof which can stimulate a distinct immune response.
D. The most effective antigen are small simple amino acidcomplexes.
E. Bacteria do not contain antigens.

4. All of the following are defensemechanism employed by vertebrates except

A. negative test, as in, not findingself-markers.

B. change in chromosomal composition

C. positive test to recognize presence ofnonself-markers

D. specific antibodies.

E. skin and mucous membranes asbarriers.

5. The immune system depends on thefunction of ______, which include several types of phagocytic cellsand two kinds of lymphocytes.

A. platelets

B. leukocytes

C. target cells

D. erythrocytes

6. In comparing the kidneys of marine andfreshwater fish, which of the following is not true?

A. Freshwater fish fluids have a greaterosmotic concentration.

B. Marine fish excrete many of the divalentcations through the anus.

C. The flow of the filtrate is faster inmarine fish.

D. The urine is less concentrated infreshwater fish.

E. Marine fish drink greater quantities ofwater.

7. The kidneys of which one of thefollowing are so highly efficient that it never has to drink waterand can obtain all of its water from its food?

A. desert lizard

B. camel

C. pocket moue Perognathus

D. kangaroo rat

E. gerbil

8. Select the incorrect statement regardingthe temperature of the immune system.

A. Interleukin-1 is a pyrogen, which cancause the neurons in the hypothalamus to raise the body'stemperature, producing a fever.

B. Fevers cause the liver and spleen tostore sodium and potassium which bacteria need for their growth andmetabolism.

C. Fevers above 105 Â° F can be fatal.

D. Fever contributes to the body's defensesby stimulating phagocytosis.

E. When macrophages encounter invadingcells, they release interleukin-1, which is carried to the brain bythe circulatory system.

9. Which of the following is not amechanism to prevent polyspermy?

A. change in membrane otential of egg

B. vitelline envelopes lift off surface ofegg

C. sperm nuclei degraded inside eggallowing one to fuse

D. cortical granule enzymes remove spermreceptors from egg

E. altering composition of exterior eggcoat

10. Which of the following cells have anincorrect function?

A. neutrophil---important antigenpresenting cell

B. eosinophil---important to theelimination of parasites

C. mast cell---releases histamine

D. macrophage---phagocytic cell

E. monocyte---precursor of macrophage

11. Which part of the antibody moleculedetermines its role in the immune response?

A. disulfide chains

B. the entire molecule

C. the stem of the Y

D. the heavy chains

E. two arms of the Y

12. Select the incorrectly matchedimmunoglobin (Ig)subclass and a fact about it.

A. IgG---found in the plasma and secretedin secondary response

B. IgM---first secreted during primaryresponse

C. IgE----promotes the release ofhistamine

D. IgA----found in saliva and motherÃ¢â¬â¢smilk

E. IgD----serve as receptors on thesurfaces of T cells

13. _______ are the source ofantibody-producing cells.

A. B cells

B. Plasma cells

C. Monocytes

D. Macrophages

E. T cells

14. Select the incorrect statementregarding the inflammatory response.

A. The alarm signals can be histamines orprostaglandins.

B. The inflammation response is a localizedspecific response to infection or injury.

C. Neutrophils spill chemical in the areaof injury or infection that kills bacteria, tissues, andthemselves.

D. Tissue can exhibit edema from thedilation of the blood vessels in the area of injury orinfection.

E. If a cell is injured or infected, itreleases chemical alarm signals that promote dilation of bloodvessels at the injury site.

15. Reproduction in reptiles compares toreproduction in birds in that

A. both newly hatched birds and reptilesrequire parental care.

B. the eggs of both birds and reptiles haveprotective shells.

C. birds are oviparous, reptiles areovoviviparous.

D. both exhibit external fertilization.

E. male or female reptiles reproduceasexually; birds do not.

16. ______diseases are those abnormalconditions in which the bodyÃ¢â¬â¢s defensive cells fail to make theself versus the nonself distinction correctly, and attack thebodyÃ¢â¬â¢s own tissues.

A. Cancerous

B. Septic

C. Autoimmune

D. Nondiscrete

17. Most marine invertebrates areosmoconformers. This means that

A. the osmolality of their body fluids isequal to that of the osmoregulators who inhabit the sameenvironments.

B. the osmolality of their body fluidsfluctuates between being hypertonic to hyportonic depending on theseason and the tides.

C. the osmolality of their body fluids isnot the same as their seawater environment

D. the osmolality of their body fluids isthe same as their seawater environment and that their bodiescontain the same solutes as their surroundings

E. the osmolality of their body fluids isthe same as their seawater environment

18. Which of the following determinesgenetic (male or female) in mammals?

A. the acrosome of the sperm

B. the temperature of the developingfetus

C. the SRY gene on the Y chromosome

D. the genetic makeup of the parents

E. the Y chromosome contained in theegg

If you can please explain why and how yougot the answer. 5 star rating for correct answers only, I have theanswer sheet, and just want to double check.