BIOC312 Lecture Notes - Lecture 6: Thioester, Malate Dehydrogenase, Symporter

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Takes place in the mitochondria in eukaryotes or the cytosol in bacteria. Energy of the osidation reactions is largely conserved as reducing power stored in coenzymes. Main coenzymes: nad+ nahd, ubiquitone (q) ubiquinol (qh2) lomoarcpsd. Pyruvate translocase transports pyruvate into the mitochondria in symport with h+, where it is coupled to an enzyme a and is decarboxylated and oxidized by huge enzyme complex pyruvate dehydrogenase. This process uses a little energy and concentration gradient of protons is required provided by the citric acid cycle. For each acetyl coa that enters; 2 molecules co2 released, coenzymes. Nad+ and q are reduced, 1 gdp (or adp) is phosphporylated and initial acceptor (oxaloacetate) is regenerated. Steps: oxidative decarboxylation of pyruvate to acetyl coa (in the pyruvate dehydrogenase complex). Nadh is formed: addition of acetate group derived from acetly coa to end product of citric acid cycle (oxaloacetate), yielding citrate (6c tricarboxylic acid).

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