BIO304H5 Lecture Notes - Lecture 2: Xfp Transceiver, Fluorescence, Polyadenylation
Document Summary
Fluorescent proteins: rather than simply fluorescent dyes, we now use fluorescent proteins, such as gfp; it"s from a gene meaning you can express this protein. Gfp is a soluble protein meaning that it can enter into cell membranes; effective at labelling neurons. You can even stick gfp to other proteins, for example one that"s involved in the formation of microtubules therefore, anywhere you find fluorescence, that is where a microtubule is found. There has since been much advancement in fluorescence in that we now have multiple fluorescent colours: pros of having multiple colours: the labelling of multiple proteins simultaneously in one cell, for example. A method in making neurons fluoresce thru fluorescent proteins. This method relies on cre recombinase enzymes excising areas of dna between lox sites, or can rely on inversion. Using dsdna with a very strong promoter (a promoter that will strongly drive transcription) past polya sequence.
