BIO372H5 Lecture Notes - Lecture 6: Sister Chromatids, Dna Replication, Chromosome Segregation

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hassan_rizvi96

20 Feb 2020

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Functions of homologous recombination in bacteria and eukaryotes. Homologous recombination has additional functions in eukaryotes. Cells with defects in recombination are hypersensitive to dna damaging agents. Animals carrying mutations that interfere with recombination are predisposed to certain types of cancer. Many types of cancer caused by damage to dna. At meiosis, recombination is required for proper chromosome pairing and for maintaining the integrity of the genome. At meiosis, the recombinant re-shuffles genes between the parental chromosomes. Variation in genes to the next generation. Cells go on to form egg and sperm cells for mating. Includes dna replication followed by two rounds of chromosome segregation (nuclear division) Results in a reduction of dna content from diploid cells to haploid cells. Before dna replication, cells must be diploid (2n) Containing two copies of each chromosome maternal and parental origin. During dna replication, chromosomes are duplicated to give a total dna content of 4n.

Related Questions

Multiply questions here

Q2. What is the consequence of chromosome inversion on meiosis? (work on a zygote with one normal chromosome and one chromosome with an inversion)

Q3. Search the web or read the textbook. Find a disease that is caused by translocation. What kind of translocation causes the disease?

Q4. Briefly explain how downâs syndrome or other trisomy is generated (3 sentences should be enough for you to explain this clearly)

Q5. I mutated a culture of wild type E. coli cells and wanted to isolate mutants that are unable to synthesize uracil (a nitrogen base in RNA). How can I achieve my goal? (try to finish your answer in 2-3 sentences)

Q6. For the ura- mutant isolated in Q5, I want to convert it to URA+ by transformation. Please tell me how I can achive this transformation with 3 SIMPLE sentences

Q7. What is the difference and relationship between an Hfr cell and an F+ cell?

Q8. I want to set up a mating between an Hfr strain and an F- (ade- his- bio-) strain and detect bio+ recombinant. Please design the experiment for me. (What genotype for Hfr do you need to choose? How will you select your recombinant? etc).

Q9. I want to use phage T2 to transducer the wild type ADE2 gene from the wild type E. coli strain to an ade2- E. coli mutant. How can I do this experimentally? (please briefly describe the approach, not experimental details such as incubation for 10 min, etc).

Q10. We talked about recombination in both eukaryotes and prokaryotes. Are there any differences in these two recombination ? (what are they if there are?)

Q11. Whose (or what) experiment proves that DNA is the genetic material?

Q12. What knowledge did Watson and Crick use in constructing the DNA model?

Q13. How does the double helix model support the three major functions of genetic materials?

Q14. What is the major chemical force that holds DNA and histone together? Hydrogen bonds

Q15. If I label E. coli cells in an N15 medium overnight and chase cells in an N14 medium for 2 generations, what fraction of cells still carry the N15 label?

Q16. If a 1000 kilobase fragment of DNA has five evenly spaced replication origins and DNA polymerase moves at 1 kilobase per second, how many seconds will a cell take to produce two daughter molecules?

Q17. DNA polymerases catalyze the polymerization of nucleotides. Use the formula for enzyme catalyzed reaction (see the example) to describe DNA polymerases. Example: Enzyme Substrate A + substrate B -------------------ï product c + product D Q8. During DNA replication, what is the direction of the growing strand?

Q18. For DNA synthesis in cells, why are there a leading strand and a lagging strand?

Q19. The following is the DNA sequence of a template strand. If the primase begins to synthesize primers from the middle âgâ, what will the primer sequence be? (assuming the primer can be 8 nucleotides long) 5â GACTCTAgGACTCCT 3â

Q20. Both DNA polymerase I and DNA polymerase III synthesize DNA during DNA replication. Please state the different roles they played during DNA replication.

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Chapter 10

1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcherâs key experiment?

2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?

3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?

4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?

5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?

Gene	Methylation levels
1	++
2	+++++
3	+++
4	++
5	+

What are the characteristics of the 3 main DNA forms?

Chapter 11

What are the different types of chromatin?

What are the structures and important roles for telomeres and centromeres?

What are the differences found between eukaryotic chromosomes and mitochondrial?

Chapter 12

Explain each of the different models of replication.

If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?

Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.

What are the functions of the different DNA polymerases found in eukaryotic cells?

Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)

What is the Holliday model of recombination and what are the necessary steps?

Chapter 13

What are the different types of RNA and what roles do they play?

Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.

Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.

Chapter 14

What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.

What is alternative splicing and what role does it play in the cell?

How is ribosomal RNA processed after transcription?

How do siRNA and miRNA work, describe/draw out the process in detail.

Question 11 options:

A condition characterized by the growth of endometrial cells in areas other than the endometrium is called

Question 12

A standard screening test for cervical cancer is called

Question 13

The period of prenatal development in which the basic parts of the organ systems are formed is the

Question 14 options:

Detection of

Spell check is used by pregnancy test kits to detect the early stages of pregnancy.

Question 15

The actual gene sequence governing the expression of a trait is called a

Spell check , whereas the physical expression of this trait is the PHENOTYPE , which can also be defined as the physical expression of a gene.

Question 16

Down syndrome is a disorder where a person has an chromosome 21.

Question

A picture of all chromosomes taken in metaphase and used for analysis is called a

Question 18

A pregnancy where the fertilized egg implants in an area other than the uterus is called

Question

The basic building blocks of DNA are called

Question 20

Monosomy of the X chromosome characterized by a short stocky neck, underdeveloped secondary sexual characteristics, and infertility is called

syndrome.

Question 21

A deletion of the short arm of chromosome 5 with a typical cry that resembles a meowing cat characterize

Question 22

A serious psychological disorder characterized by the fear of becoming obese is called

Question 23

Diffusion of gases between capillary beds and tissues is called respiration.

Question 24

The destruction of alveoli by irritants such as cigarette smoke and agricultural dust is found in a disorder called

Question 25

Of the three types of skin cancer which are the most likely to metastasize

Question 25 options:

Melanoma

Squamous cell carcinoma

Basal cell carcinoma

All of the above have equal ability to metastasize

Question 26

Cancer of this organ/gland causes the most deaths annually

Question 26 options:

Lung

Breast

Prostate

Bladder

Question 27

The correct order of stages of the cell cycle that a new cell will go through immediately after being produced by mitosis is

Question 27 options:

S, G1, G2, M

G1, G2, M, S

G1, S, G2, M

M, G1, S, G2

Question 28

What is the purpose of DNA replication

Question 28 options:

To copy the information in a gene so that extra protein synthesis can be done

To make an RNA copy of the gene

To provide the cell with extra DNA in case the original gets damaged

To ensure that two cells produced by mitosis have the same genetic information as the parent cell

Question 29

Which of the following is not a function and/or advantage of mitosis

Question 29 options:

It can repair damaged tissue

Growth takes place through mitosis

It can replace old or damaged cells

All of the above are functions and/or advantages

Question 30

Which stage of the cell cycle occurs just after mitosis

Question 30 options:

Prophase

Save

Question 31 (1.5 points) Question 31 Unsaved

DNA replication is completed by the end of

Question 31 options:

Prophase

S phase

Question 32

Which of the following is NOT true of DNA

Question 32 options:

Generally double stranded

Stores the cell genetic information

Replicated by DNA polymerase

Contains A, C, U, and G

Question 33

These cell may enter G0 of the cell cycle permanently

Question 33 options:

Hepatocytes

Transformed cells

Neurons

Cells in the digestive system

Question 34

When a primary spermatocyte, goes through meiosis, how many mature sperm will be produced

Question 34 options:

Question 35

When a primary oocyte goes through meiosis, how many mature eggs will be produced

Question 35 options:

BIO372H5 Lecture Notes - Lecture 6: Sister Chromatids, Dna Replication, Chromosome Segregation

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