BIOB11H3 Lecture Notes - Lecture 4: Guanosine, Restriction Site, Rna Splicing
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Lecture 4: transcription ii: hnrna transcription and processing. The larger the rna, the farther it travels during centrifugation and the closet to the bottom of the tube it ultimately lies. The absorbance (blue line) indicates the total amount of rna in different regions of the centrifuge tube, whereas the red line indicates the corresponding radioactivity. It is evident that most of the newly synthesized rna is very large, much larger than the stable 18s and 28s rrnas. It is evident that the large hnrnas have been processed into smaller rna products. 11. 16 initiation of transcription from a eukaryotic polymerase ii promoter: a) nucleotide sequence of the region just upstream from the site where transcription is initiated in. The tata box is indicated by the blue shading. Many eukaryotic promoters contain a second, conserved core promoter element called the initiater (inr), which includes the site where transcription is initiated (shown in orange).