BIO130H1 Lecture Notes - Lecture 7: Helicase, Primase, Phosphodiester Bond
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BIO130H1 Full Course Notes
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Bio130 lecture 7 midterm notes: dna helicase unwinds the dna; the initiator proteins help the helicase to bind to the origin. Eukaryotes have many: the primase binds to the helicase; it is now called a primosome, the rest of the replication machinery binds. Most thermally stable when dna is complementarily base pairing closed. After the helicase, single stranded proteins prevent the strands from hydrogen bonding and they keep the strand separated. The dna helicase is always on the lagging strand, which acts as the strand: the helicase has 6 subunits, the helicase therefore is a quaternary structure. The nucleotides can only be added on a 3" end: single strand binding proteins keep dna strands separated. Ssdna binds and prevents the strands from forming hydrogen bonds: rna primers are synthesized by primase which activate the activity of the dna polymerase. Primase makes the rna primer in a 3" to 5" direction along the template strand.
