BIO130H1 Lecture Notes - Lecture 10: Polymerization, Atp Hydrolysis, Depolymerization
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BIO130H1 Full Course Notes
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A red blood cell poked with laser tweezers returns to its original shape due to structural support provided by the cytoskeleton. Three types of filaments form the cytoskeleton: microfilaments (aka actin filaments) Actin filaments look like two protofilaments in double-stranded helix. Microtubules look like 13 protofilaments that form a hollow cylinder. The light microscope has a resolution limit: due to diffraction. Bending of light/waves as it passes through obstacles. ~250nm (microtubule shave a diameter of 25nm) o o o o. A technique used to determine the location of proteins within the cell. An antibody is used which binds specifically to the protein of interest (primary antibody) A second antibody binds to the first antibody and is covalently tagged with a fluorescent molecule (secondary antibody) A fluorescence microscope is used to excite the fluorescent molecule and visualize the light emitted (cheaper with two antibodies instead of prim. w/ fluor. ) Can see cross-section with electron microscope to see individual components.
