BIO130H1 Lecture 6: Lecture 6 Translation
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BIO130H1 Full Course Notes
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Document Summary
Genetic code: codons read as mrna triplets, encoding all 20 aa, redundancy multiple codons for most aa, first 2 bases are the most important, codons reads 5" to 3" end. Reading frame- phase in which nucleotides are read in sets of 3 to encode a protein mrna molecule; can be read in any 1 of 3 reading frames but only 1 will give required protein. Aa matching codon/anticodon pair is attached at 3" end of trna at acceptor stem. Have 20 trnaase (one separate for each aa) To ensure fidelity of genetic code: aminoacyl- trna synthetase- covalently couple aa to appropriate set of trna recognition and attachment of correct aa depends on this enzyme (quality control) Similar to dna polymerase (2 binding sites synthesizing and editing) Synthesizing site adds appropriate aa for the right trna. Sometimes mistakes occur (wrong aa is attached) . Attachment of aa to 3" end of trna is coupled to energy releasing hydrolysis of.
