BCH210H1 Lecture Notes - Lecture 7: Sickle-Cell Disease, Extrachromosomal Dna, Recombinant Dna

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Stavroula Andreopoulos

Document Summary

Genetic engineering molecular biology: modifying proteins by modifying their genes, genetic engineering has revolutionized the study of protein structure/function, allows us to ask sophisticated questions about the role of any one amino acid in a protein. Insert the gene in an expression vector" (e. g. dna plasmid) Mother of all plasmids : ecori, bamhi, etc. Restriction sites: ori origin of replication (needed to make more dna) A protein expression plasmid: ecori restriction sites, origin of replication, ampicillin resistant gene same as pbr322, also has a promoter gene allows us to start making. Rna polylinker cloning site: thousands of restriction enzymes can be engineered that do not already exist in the plasmid. Linear dna cut with a restriction enzyme want to put it into the plasmid but it has a blunt end so it cannot enter: add a linker using dna ligase. Ligase keeps adding more and more linkers that we don"t need if we treat it with.