BCH210H1 Lecture Notes - Lecture 7: Ribosome-Binding Site, Fusion Protein, Lysis

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Stavroula Andreopoulos

Document Summary

Lecture 7 outline: genetic engineering of protein. If protein occurs naturally in low amounts, you have to use recombinant techniques to increase amount of protein for study in culture (isolation) or tissue- specific cells. Sdm: changing dna to change codon to another amino acid: biotechnology industry and life sciences is larger than automotive industry in. Genetic engineering: basic approach: clone the gene of interest from genome or cdna from mrna. Insert the gene in an expression vector" (e. g. , Insert ( transform") the plasmid into bacterial host: grow bacteria; they express the protein. If you have a bacterial cell, you can pcr the particular gene, ligate it into a vector. Plasmids are used to clone genes: plasmids: naturally occurring extra-chromosomal. Dna: circular double-stranded (ds) dna, can be cleaved by restriction enzymes, leaving. Sticky ends : can be constructed by linking new dna fragments to the overhangs of plasmid, these recombinant molecules can be replicated many fold in bacterial host.