CSB328H1 Lecture Notes - Lecture 5: Transcriptional Regulation, Blastocoel, Fate Mapping

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Acquisition of a cell fate: on its way on becoming a specialized cell, it has to make a decision on what to become and this decision is made in two steps. Test for specification: isolated explants (prospective epidermis) You have early frog embryo (early gastrula) when the formation of the mesoderm begins. Cut out the prospective epidermis form the embryo and put it into a dish (isolated form the rest of the embryo) and see what happens to the piece of tissue. The piece of tissue develops into epidermis just on its own (does not need the rest of the embryo) The fate the piece of tissue acquires is what you expect from the fate map (predictable) This means that the epidermis fate is already specified in the early gastrula stage. In this case, the acquired fate does not equal the normal prospective fate. The neural fate has not been specified at this particular stage (early gastrula.

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