MGY299Y1 Lecture : Notes taken during lecture
19 Dec 2011
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On the way to a 16s rrna gene sequence for your unknown: verify api identification of washroom sample by 16s sequencing. 5s wouldn t have enough information to distinguish need highly. Treat sample with protinease k to rid of excess protein. First half of molecule: colour coded to indicate degree of conserved-ness, use more conserved sequences for pcr primers, variable regions useful because distinguish btw families etc. Run pcr product on agarose gel: to verify/detect 800 base pair fragment. Compare sequence to database blast: determine identity of organism. 3 domains of life bacteria, archea, eukaryotes. If have fungal contaminant in sample, would pick it up because primers would bind to its rrna coding dna. Bacteria and archaea more closely related than either to eucaryotes: all life from one source, but eukaryotes can share features more closely with archaea than bacteria. Get mixture of chains that have stopped at different places.
