MGY378H1 Lecture Notes - Ubiquitin Ligase, E2F, Retinoblastoma Protein
3 Jun 2013
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Adenovirus linear dsdna genome inverted terminal repeats at ends five prime end covalently linked protein called terminal protein . Early proteins scattered over genome some transcribed on one strand, tend to have own promoters each of early genes can be spliced different ways e1a proteins are a group, related but different. Late proteins all come from common promoter also have splicing to get single messenger rna spliced, tripartite leader sequence. Rna: make viral proteins needed for dna replication , e1a protein two expressed early three more expressed later in infection. Part of rb works in suppressing gene expression is by recruiting h-dac histone d acetylase acetylation of histones or histone tails determines whether chromatin active and can be transcribed or not . E1a can efficiently bind to rb same place that e2f binds blocks rb binding in presence of e1a whereh e2f active and turns on genes promoting s phase progression.
