PCL102H1 Lecture Notes - Lecture 9: Reverse Transcriptase, Metastability, Complementary Dna
12 Feb 2016
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Week #3: lecture 9: microarrays every cell has an unlimited potential; gene expression controls this potential. All cells have the same genes inside them (dna) but only some genes are turned on ; this creates diversity by limiting the genes that are turned on ; gene expression is continuous. Monocyte have both genes but make myosin mrna and not immunoglobulin mrna. Lymphocyte has both genes but make immunoglobulin mrna an no myosin mrna. Some things that can change gene expression: expressed examine gene expression. When you compare gene expression in two cells many/most genes are not differentially. Differences in gene expression can reflect difference in underlying cellular state. Other applications include comparison of dna copy number, gene amplification/deletions. Target protein is given a chance to bind to the candidate drug. Asses transcriptome at a particular moment in time (snapshot) Dna oligonucleotides is a solid support (slide)
