Anatomy and Cell Biology 4429A Lecture : Aging
Document Summary
Developed many cell lines - developed cell lines for salk to grow his vaccine on. Over a period of days, you would start with x amount of cells. You can measure how many times the population goes through mitosis. Eventually, after six months, the growth slows down. The cells that started off in early passage, by the time they get to late passage, they have enormous cell bodies. The resulting population of cells is entirely female. If this was inept tissue culture, the female cells should stop as well. Property of the cells, not of the tissue culture being used, so it was a real effect. As cells progress through a lifespan, these inhibitors begin to increase. These cells don"t die, they just don"t go into mitosis. Cells also remember how old they are - if you freeze cells for a couple of years and replate them, they know where they are in their lifespan.