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Lecture

Differences between Prokaryotic and Eukaryotic Transcription

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Department
Biochemistry
Course
Biochemistry 2280A
Professor
Eric Ball
Semester
Fall

Description
Differences between Prokaryotic and Eukaryotic TranscriptionDifferences b prok and euk1 Three RNA polymerase in eucaryotes Bacteria have 1 typePol I transcribes most rNA genes Pol II makes mRNAs Pol III makes tRNAs 5S rRNAThree polymerases are very smilainr in strucutre and they differ enough that they recognize diff promotors It provides another level of complexity of regulation on euk gene expressionNo operon in euc We talked about lac and tryp operon where multiple protein encoding gene is transcribed in same message This doesnt happen in euk each gene has own promoter and makes single protein producteach gene is transcribed as a single unit monocistronic in cotnrast to polycistronicmultiple protein encoded on same mrna in bacteria Promotor structure is quite different How does euc rna polymerase recognize promoteorwe have protein encoding genestart site there upstream of that is TATA box Looks a bit like 10 but its not Its about 20 bases or so from start site for human That tata box is recognized by protein complex called TFIID bind
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