Biochemistry 2280A Lecture : Differences between Prokaryotic and Eukaryotic Transcription

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orchidotter238

26 Dec 2011

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Differences b/ prok and euk: three rna polymerase in eucaryotes. Three polymerases are very smilainr in strucutre and they differ enough that they recognize diff promotors. It provides another level of complexity of regulation on euk gene expression. We talked about lac and tryp operon where multiple protein encoding gene is transcribed in same message. Each gene has own promoter and makes single protein product. Each gene is transcribed as a single unit (monocistronic; in cotnrast to polycistronic(multiple protein encoded on same mrna) in bacteria) We have protein encoding gene start site there, upstream of that is tata box. Looks a bit like -10 but its not. Its about 20 bases or so from start site (for human). That tata box is recognized by protein complex called tfiid (bind) (trascritpion factor ii d). It has part of it, tata binding protein that reocgnizes tata element.

Related Questions

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

1. How is the structure of eukaryotic DNA and chromosomes related to how transcription occurs?(Hint: histones and winding is important)

2.Compare RNA polymerase in bacteria to the RNA polymerase in eukaryotic cells? What are the structural differences? How does this relate to how each works?

3.Compare and contrast the promoters found in prokaryotes and eukaryotes.

4.How is initiation started in eukaryotes?

5.What are transcription factors and what role do they play?

6.How are elongation and termination similar in eukaryotes & prokaryotes? How do they differ?

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

Biochemistry 2280A Lecture : Differences between Prokaryotic and Eukaryotic Transcription

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