Biochemistry 3382A Lecture Notes - Lecture 9: Lac Operon, Lac Repressor, Catabolite Repression

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 3382A
• Professor: Derek Mc Lachlin

Document Summary

Ribozymes are rna segments that carry out enzymatic activities in the absence of proteins (i. e. rnase-p, peptidyl transferase, etc). Abzymes are antibodies that are raised to bind the transition state of a reaction of interest. Ribozymes: a crystal structure of a 50s subunit from h. marismortui shows large amounts of proteins (for structural scaffolds) and rna components. The active site of ribozymes is where the peptide cross- linking occurs and is composed of primarily rna components. Cleavage can occur without the aid of any other protein. Requirements: looped single strand with a regular complementary strand bound to it. The cleave occurs in the complementary strand and the process can be used in vitro to generate two strand constructs of a specific length. Ribosomal rna provides the structural and functional foundations for ribosomes. Rna and 1/3 protein. rrna serves as a scaffold for ribosomal proteins. The svedberg unit (s or sv) is a non-si unit for sedimentation coefficient.