Biology 2581B Lecture Notes - Lecture 7: Wild Type, Purine, Base Pair

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orangealligator209

26 Jan 2016

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Can think of this as cell to cell or organism to organism. Mutant something has happened on the genomic level that caused a change of phenotype. Keep in mind though some things have iden@cal dna but look di erent because of the environment. Can test which yeast strain will grow in absence in a certain thing form of tracking. To de ne what is the wild type versus mutant. We think of whatever the norm is to be the wild type. Some depend on the condi@ons youre exposed to change might be bene cial or not depends on interac@on. Altered molecules need assays need to track. Anything you can come up with and track you can use as a phenotype to track popula@on to study it. Changing one base pair somewhere in the sequence (not base, but base pair will have consequences on the compllementary strand) Top and bouom on right side have same change.

Related Questions

Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).

Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium, however, they can grow on medium supplemented with adenine.

All three strains are homozygous for the underlying alleles.

When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium and require adenine supplementation;

when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium and do not require adenine.

A. What conclusions can you make about the alleles of mutant strains 1, 2, and 3 and their relationships with each other?

B. What phenomenon is occurring in the cross between mutant strains 1 and 3?

After crossing the F1 generation of the cross between mutant strains 1 and 3, you count and determine the phenotypes of 1,000 colonies (here a colony is equivalent to an individual): 563 colonies that can grow on minimal medium; 437 colonies that require adenine supplementation.

C. What genotypes lead to the phenotypes of the F2? What phenomenon is occurring in the F2 generation

turquoisemink806

. You were given a strain that one friend claims requires threonine and another friend claims that it does not. You are sure you can tell the difference so you streak the unknown strain on a minimal plate. a) If it didn't grow, could you conclude that it does in fact require threonine? Why or why not? b) If not, how would you modify your experiment to allow a conclusion? After you do the experiment correctly you are still confused because the strain can make small colonies without threonine, but it seems to make bigger colonies when threonine is present. c) You do a growth curve to quantitatively demonstrate a growth difference between the WT and the mutant strain. Predict/draw what you would expect the growth of the WT and mutant strains to look like in minimal medium. Predict/draw what you would expect the growth of the WT and mutant strains to look like in minimal medium + thr. d) Assuming you demonstrated a partial requirement for threonine in this strain, what kinds of mutant proteins could cause this phenotype (list 2)? Explain how the mutation could lead to a partial requirement?

fuchsiasea-lion291

Cell Biology (#1-5)

1) What does a non-synonymous DNA mutation cause?

a) No amino acid change

b) A change in the reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

2) What does a non-sense DNA mutation cause?

a) No amino acid change b) Alters the normal reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

3) If you sequence the genome of 100 cells from different tissues of the same multicellular organism. How do you expect the sequences to be?

a) 10% identical c)100% identical

b) 50% identical d) 100% different e) will depend on the tissue selected

4) Multicellular organisms are comprised of a variety of cells with different morphologies and different functions. Which is the underlying mechanism that leads to the generation of diverse cell in one organism?

a) The expression of all genes in all cell types

b) The duplication of the genome in certain cell types

c) The elimination of specific group of genes from different cell types

d) The differential expression of particular sets of genes requires for a particular cellular phenotype

e) All of the above

5)You are growing bacteria which can use lactose and glucose. The culture medium has lactose, which induces transcription of the enzymes required to use lactose. If you add glucose to the medium. What do you expect to happen?

a) The bacteria will keep using lactose

b) The bacteria will shut down the Lac operon and use glucose

c) The bacteria will stop growing

d) The bacteria will die

e) The bacteria will use both lactose and glucose

salmoneagle868

Biology 2581B Lecture Notes - Lecture 7: Wild Type, Purine, Base Pair

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