Biology 1202B Lecture Notes - Lecture 13: Chloroplast, Nonsense Mutation, Reading Frame

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Prokaryotes- mrna produced by transcription is not confined within a nucleus and is therefore available immediately for translation. Eukaryotes- mrna produced by splicing of pre-mrna first exits the nucleus and is then translated into cytoplasm. Translation- mrna associated with a ribosome and trna (transfer), which brings amino acids to the complex to be joined, into the polypeptide chain. Overview: ribosomes assemble amino acids into a polypeptide chain trna molecule with an aa bound to it is entering the ribosome on the right. The anticodon on the trna will pair with the codon in the mrna. Its aa will then be added to the growing polypeptide that is currently attached to the trna in the middle of the ribosome. As it assembles a polypeptide chain, the ribosome moves from one codon to the next along the mrna in the 5"3" direction trna structure trna brings aas to the ribosome for addition to the polypeptide chain.

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