Aim: To study the effect of temperature on enzyme reaction rate
Protocol: End point Assay method
3.9ml Tris HCl pH 9.0
1.0ml substrate solution
At T=0 , add 0.1ml alkaline phosphatase (enzyme)
At T=4min, add 2ml 2N NaOH
Read absorbance at end of 4min at 410nm.
Repeat 3 times at each temperature
Observation (End point assay method)
Sr. No TEMPERATURE (˚C) Observation (Absorbance @ 410nm) t= 4 min
1 2 3
a. For each treatment, plot product concentration vs. time.
b. For each treatment, determine reaction rate using the graphical method and put the