Physiology 3140A Lecture Notes - Lecture 5: Ap2 Adaptor Complex, Dynamin, Heterotrimeric G Protein
3 Jul 2017
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Class a: 2 adrenergic receptors, lpa1 receptors: rapidly dissociate from -arrestins upon endocytosis, -arrestins recycles to cytosol, receptors trafficked to endosomes where ligand is dissociated, receptor is dephosphorylated and recycled to the pm rapidly. Mainpoint: what defines class a vs. class b is the stability of the receptor with the -arrestin complex, not how they are recycled. Internalization: temporarly gpcr removal into interior of cell so that they are no longer available for ligand binding and are unable to interact with heterotrimeric g protein (no longer signal) Gpcr endocytosis experiment: 0 min indicates no agonist yet, -arr in resting state and mostly cytosolic (see gfp, ago(cid:374)ist added (cid:272)a(cid:374) see that -arr moves to. Pm: overlay indicates interaction between lpa re(cid:272)eptor a(cid:374)d -arr (yellow) at the surface, this is qualitative analysis it suggests i(cid:374)tera(cid:272)tio(cid:374) (cid:271)et(cid:449)ee(cid:374) the t(cid:449)o (cid:271)ut does(cid:374)"t guarantee it is happening i n. Roles of -arrestin: regulate the desensitization of.
