BIOL 1000 Lecture Notes - Lecture 2: Methylene Blue, Pressure Measurement, Pipette
12 Mar 2019
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Document Summary
The cumulative oxygen production (ml o2) of the aquatic plant, cabomba caroliniana under white light, is used to determine the photosynthetic rate (ml o2/g/hour). Experimental tubes 1 and 2 contained approximately the same amount of aquatic plant whereas the control tube underwent the same processes without the aquatic plant. All tubes were experimented twice with pond water, and white light under room temperature (23 c). A manometric apparatus is a closed system that does not allow any gases to enter or escape, and was used to record the oxygen production. This was measured through the change in pressure shown by the displacement of methylene blue in a pipette. The photosynthetic rate is the cumulative oxygen production rate which is determined by the slope (ml o2/hour) divided by the weight (g). The cumulative oxygen production was recorded every 0. 03 hours for a total of 0. 27 hour.
