MCB 3020L Lecture Notes - Lecture 11: Antisense Rna, Autoinducer, Lux
Document Summary
Therefore we get no transcription of target genes or ativation of luxr. In a high cell density many signal molecules collect or aggregate. Only if 10 million bacteria are accumulated does the auto-inducer build up to turn on the genes for luciferase. Lux r: auto inducer bind to the promoter of the of lux genes are recruits rna polymerase sigma subunit. Rna that is complimentary in sequence to mrna and therefore base pairs with it. Blocks the ribosome binding site of mrna. (no protein will be made. ) When gene 1 and gene 2 transcribe give you two rna that are complimentary with each other and can bind together. If you flip gene 2 and the codons align as a complimentary base pairs. Gene is transcribed constituitvley but only needed if iron is plentiful. Anti-bfr gene- resulting in mrna that is anti to bfr gene. Ferric uptake regulator (fur) global regulator protein involved in iron uptake.
