BIOL-UA 21 Lecture Notes - Lecture 14: Cpg Site, Tata Box, Column Chromatography
11 Nov 2016
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Lecture 14: eukaryotic transcription: cells were lysed and the proteins were passed though ltration columns that separated them according to size, shape etc, the rst fraction would have smaller protein complexes. They took each fraction and looked if that fraction could support rna synthesis. Mixed with dna and ribonucleotides and looked if transcription occurred and translation. Each of the rna pol were different fractions and were off different sizes, and only those fractions had rna synthesis activity. The transcription by the fraction of pol i and pol. Alpha amanatin speci cally interfered with pol ii: overall structure of rna pol are similar: Eukaryotic rna pol ii has a ctd domain. (c terminal domain: contains same 7 amino acids repeated many times. ) Some of these amino acids can be phosphorylated: transcription had to be investigated in interphase chromosomes. Drosophila polytene chromosomes are visible because many copies are kept together due to replication and no separation.
