11:115:301 Lecture Notes - Lecture 14: Inner Mitochondrial Membrane, Mitochondrion, Succinyl-Coa

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Acetyl-coa = the fuel for the citric acid cycle. Two carbons of the acetyl group oxidized to co2. Most energy produced by 8 high energy electrons on nadh + fadh2. Goal take acetyl-coa and oxidize it completely. Tca takes place in the mitochondrial matrix. The mitochondrion, the citric acid cycle, and biosynthetic factory. Related to gram-negative bacteria found in the wild. Mitochondria have their own small genomes + double membrane. Intermembrane space develop protein gradient acidic. Most enzymes = dissolved in the matrix. Tca cycle oxidizes an acetyl group to co2, atp, nadh, fadh2. Pyruvate acetyl-coa (2c) + oxaloacetate (4c) citric acid cycle. Eventually the reduced cofactors yield energy in the form of atp. 1. 0 atp (or gtp) substrate level phosphorylation. 3 x nadh = 2. 5 atp each. For each glucose 2 x pyruvate. The gtp is formed by gdp-forming succinyl-coa synthetase. Can be used by nucleoside-diphosphate kinase form atp. Nadh/fadh2 electron transport chain proton gradient atp.

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