BICD 100 Lecture Notes - Lecture 16: Genetic Engineering, Forward Genetics, Congenital Heart Defect

• School: UCSD
• Department: Biol/Genetics,Cellular & Develop
• Course: BICD 100
• Professor: Deborah Yelon

Document Summary

Using model organisms to decipher roles of genes. Genes and their roles are conserved among diverse organisms. By studying model organisms, we can identify genes that may play similar roles in humans. How to decipher the role of a gene. Using forward genetics to decipher the role of a gene. By mapping the mutated gene and correlating with effects. Do any of the f3 progeny have an interesting phenotype. You want to determine if the induced mutation "m" causes heart defects. You perform 20 random pairwise intercrosses of siblings from a f2 family. If m/m homozygotes have heart defects, approximately how many of the f2 pairs would produce some progeny with heart defects. In the cases when the f2 pairs produce progeny with heart defects, what percentage of the embryos in that clutch would you expect to have heart defects. Alter a gene of unknown function, figure out resulting phenotype. Gene "knock outs" in mouse week 9 page 1.