BIOB11H3 Lecture Notes - Lecture 5: Rna Splicing, Precursor Mrna, Primary Transcript
Document Summary
Transcription mrna synthesis mrna synthesis: transcription initiation, primary transcript mrna processing, 5" capping, 3" poly (a) tail, splicing of introns, discovery of introns (split genes, spicing erros mrna (messenger rna): genetic message for translation to protein. Synthesized from pre-cursor rna (primary transcript) called heterogeneous nuclear. In eukaryotes, mrna must be processed inside the nucleus before transport to the cytosol. Rna processing starts before transcription is completed mrna synthesis and processing is tightly linked mrna processing (in eukaryotes and prokaryotes) Organization of protein coding genes prokaryote vs eukaryote using the genes involved in tryptophan synthesis. Rna polymerase ii synthesizes all eukaryotic mrna precursors: highly conserved, several different subunits. Requires general transcription factors (gtfs): general" because their binding is required for transcription to initiate at many different promoters. Pol ii recognizes promoter regions located towards the 5" side of the transcription unit. Tata box: between 24 and 32 from the transcription initiation site.
