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26 Jun 2019

The following DNA contains one transcribed region which comprises 2 exons and one 10-nucleotide intron. The transcription start site (cytosine) and branch point (adenine) are lower-case. 5â-CCCTCGAcTCGTAATGGAAAGGTGCGAGTGGGCaCAGGCGCGGGGAAAATGAGACTATTTGTAGCTGACCCTC-3â

Write the template sequence for this transcribed region. Label 5â and 3â ends. Write the pre-mRNA sequence. Label 5â and 3â ends and circle the intron (which is removed).

Using single letter amino acid abbreviations, translate the mRNA. Label amino- and carboxyl-termini. An insertion mutation adds a cytosine to the template strand between the first two codons.

Translate the new mRNA.

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Trinidad TremblayLv2

28 Jun 2019

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Related questions

Can someone please check and help me with these? Especially II and III?

I. Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:

3â TAA ATA CGT GAG GCA CTT CAA AGA CCG CAC ACT_ AAT TT 5â

5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â

a. Transcribe the DNA template into a strand of mRNA by typing the mRNA sequence below the template strand. Label the 5â and 3â end of the mRNA 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â

b. Paste the mRNA below and do all 3 reading frames. Highlight all start codons green in all the reading frames. Highlight red the stop codons which are in frame with the start codons in all the 3 reading frames if they are present.

1) 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3â

2) 5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â

3) 5â AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3â

c) In which reading frame is the ORF found? The second one. Since it has AUG.

d) Paste the mRNA showing the most likely ORF below and translate the codons. Label the 5â and 3â end of the mRNA and the N- and C-termini of the peptide.

5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â

MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP

N Terminus C Terminus

II. 3 pts. The base calling program made a mistake that you didnât catch and deleted the bolded G in the original template strand above. Paste the template DNA below and delete the G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.

5â AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A â3

MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP

III. 3 pts. The base calling program made a mistake that you didnât catch and added an extra G after the bolded G in the original template strand above. Paste the template DNA below and insert the extra G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.

5â AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A â3

MET HIS SER VAL LYS PHE LEU GLY CYS ASP STOP

2) For the hypothetical DNA sequence/gene below mark the start codon and the stop codon of the gene. TSS->

5âCATAAA| GCAAACAGGAGATGGGCCCCAAAAGCCATGAATGGGAGCGCGTATAATCATGATTTATAATCTAGG TATCTCACTGA3â

3âGTATTT| CTTTTGTCCTCTACCCGGGGTTTTCGGTACTTACCCTCGCGCATATTAGTACTAAATATTAGATCC ATAGAGTGACT5â

Terminator sequence

Promoter sequence

TSS-> transcription start site and direction of transcription

a) Which strand of the DNA shown above, the top or the bottom, is the template strand?

b) What is the sequence of the mRNA produced from this gene? Label the 5â and 3â ends.

c) What is the sequence of the protein produced from the mRNA in (b)? Label the N and C termini.

d) Generate two examples each that result in a silent mutation, a missense mutation, a nonsense mutation, and a frameshift mutation for the DNA sequence above. Show the respective amino acids below each codon. (Courier font is a font typically used for sequences.)

ochrecrab921

a) Using the template strand sequence below from a EUKARYOTE, transcribe it to mRNA, properly end modify it, and then translate it into protein. Do not worry about splicing, this is one of the rare genes with no introns. Use the single letter amino acid abbreviations when writing the protein sequence.

5â- GTTAACGCTTCAGGAGACACTTCCCGATAAGCACCATGGTGGCCCG -3â

1 2

b) If there is a transition mutation at the arrow labeled 1 and a transversion to a G at the arrow labeled 2, what will be the new mRNA and protein sequence become? Is the protein likely to be functional?