Southern blot analysis of the three genotypes found at the β-globin locus revealed a distinctive pattern of RFLP variation among the genotypes, while northern blotting reveals no difference. What results would you expect from native electrophoresis of the protein from the three β-globin genotypes?
(a) A western blot analysis performed on protein from individuals with the three β-globin genotypes shows that βAβA individuals have a single protein band, whereas βSβS individuals have no protein. βAβS heterozygotes have half as much protein as a homozygous individual.
(b) A western blot analysis performed on protein from individuals with the three β-globin genotypes detects a single protein band, so all genotypes look identical by western blotting.
(c) When a western blot analysis is performed on protein from individuals with the three β-globin genotypes, βAβA and βSβS individuals each have one distinct band, whereas βAβS individuals have both bands.
(d) A western blot analysis performed on protein from individuals with the three β-globin genotypes looks very similar to the Southern blot results. βAβA individuals have two bands, βSβS individuals have one band, and βAβS individuals have all three bands.
Answer is C because western blotting uses proteins.
Please explain the northern, southern, and western blots and why a, b, and d were incorrect. Use drawings when necessary.
Southern blot analysis of the three genotypes found at the β-globin locus revealed a distinctive pattern of RFLP variation among the genotypes, while northern blotting reveals no difference. What results would you expect from native electrophoresis of the protein from the three β-globin genotypes?
(a) A western blot analysis performed on protein from individuals with the three β-globin genotypes shows that βAβA individuals have a single protein band, whereas βSβS individuals have no protein. βAβS heterozygotes have half as much protein as a homozygous individual.
(b) A western blot analysis performed on protein from individuals with the three β-globin genotypes detects a single protein band, so all genotypes look identical by western blotting.
(c) When a western blot analysis is performed on protein from individuals with the three β-globin genotypes, βAβA and βSβS individuals each have one distinct band, whereas βAβS individuals have both bands.
(d) A western blot analysis performed on protein from individuals with the three β-globin genotypes looks very similar to the Southern blot results. βAβA individuals have two bands, βSβS individuals have one band, and βAβS individuals have all three bands.
Answer is C because western blotting uses proteins.
Please explain the northern, southern, and western blots and why a, b, and d were incorrect. Use drawings when necessary.
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Question 22 options:
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