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A child was born with very high levels of phenylalanine in her blood. However, a follow-up test showed that the child has normal alleles for phenylalanine hydroxylase. a. Propose a hypothesis to explain how the child could have high levels of phenylalanine yet have no mutation of the PAH gene. b. What test or tests would you do to show your hypothesis is correct and what would be the results?

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Patrina Schowalter
Patrina SchowalterLv2
28 Sep 2019
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. A child with very low blood oxygen levels was given a test foralpha thalassemia The test was a Northern blot test using astandard probe that binds to alpha globin mRNA. The resultingNorthern blot had three bands, indicating the child has threedifferent sizes of alpha globin mRNA molecules. One is normal size,one is larger than normal, and one is smaller than normal. Theparents of the patient are both from Mediterranean countries butthere is no reasonable chance that they are related.

a). Give one possible mutant genotype that the child might havethat would result in her forming three different sized alpha globinmRNA molecules, and explain how this genotype would also cause herto have low blood oxygen levels.

b). What test would you do to confirm your hypothesis?

c). Predict what results you would expect from your test if yourhypothesis is correct

. Carlos and Sophia have brought their child Maria to your hospital. She has lower than normal blood O2 levels and other symptoms of ß thalassemia, although those symptoms are not as severe as many cases of this disease. A Northern blot was done on the mRNA from Maria using a probe that binds to the third exon of the ß globin gene. The Northern blot showed a band of normal size and density. Maria’s parents are of Hispanic (Mediterranean) origin, and they do not know if they may be distantly related.

a. Give one hypothesis for what Maria’s genotype might be that explains her symptoms and also the Northern blot results.

b. Design a test or tests that would prove that your hypothesis in a is correct.

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d. Explain to Carlos and Sophia (who are not scientists) how the results of your test(s) confirm your hypothesis.

Please help me with this question. This is a question from Genetics class:

A child has been admitted to your hospital with low blood O2 levels. A test for β thalassemia (a Northern blot) was done using a standard probe for β globin mRNA. This blot gave three different bands, indicating the patient has mRNA of three different sizes: one larger than normal, one smaller than normal, and one normal. The parents of the patient are both from Mediterranean countries but there is no reasonable chance that they are related.

a. Devise a hypothesis for the genotype of the child that explains how their genotype gives β thalassemia and also three different sizes of mRNA.

b. Devise a test (or tests) that will determine if your hypothesis is correct.

c. What results would you predict your test would give if your hypothesis is correct.

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