Question

A strain of E. coli has the following mutant genotype: lacZ-gyrA- hisA- (mutations in all three genes). Indicate by a yes or noin the answer space whether the strain would grow on minimal mediawith the carbon sources (maltose, lactose or glucose) andsupplements as indicated below. Lactose and maltose are sugars andhistidine is an amino acid. One of these three mutations leads toresistance to an antibiotic.

1. Minimal + maltose + histidine
2. Minimal + lactose + histidine
3. Minimal + glucose + histidine
4. Minimal + lactose + nalidixic acid
5. Minimal + glucose + histidine +nalidixic acid