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carminedog609Lv1

28 Sep 2019

What was the purpose of adding methotrexate to the cellsexpressing a genetically-engineered Dihydrofolate Reductase enzymein the mitochondrion-targeting experiment? What did the experimentdemonstrate in terms of the location of the site(s) oftranslocation of proteins into mitochondria?

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Lelia LubowitzLv2

28 Sep 2019

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Related questions

For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume the following:

- Each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found.

- Only targeting sequences possessed by each protein are those listed.

a) A cleavable ER-signal sequence and a stop transfer sequence (answer is not ER)

b) A peroxisomal sequence and a lysosomal signal sequence (answer is not lysosome)

c) No sorting signal (answer is not ER)

d) Multiple start and stop sequence signals (answer is not membrane of ER)

orchidkangaroo356

(a) Mitochondria (b) Endoplasmic reticulum (c) Nucleus (d) Lysosome (e) Endosome (f) Peroxisome (g) Golgi apparatus

_____ quality control of mRNA

_____ location of oxidative phosphorylation

_____ responsible for detoxifying organic molecules (liver cells)

_____ houses and protects genetic material

_____ responsible for modification and sorting of proteins and lipids

_____ location of ATP synthesis

_____ responsible for sorting endocytosed materials

_____ site of degradation and digestion

_____ responsible for oxidizing toxic molecules

_____ location of lipid synthesis

_____ location of hormone synthesis (adrenal cells)

B. Which organelle(s) from the above list communicate(s) with other organelle(s) through the use of vesicular transport? ________________

C. Which organelle(s) from the above list receive proteins made in the cytosol?___________________

D. Through which organelle(s) must proteins pass to reach the organelle(s) which do not receive proteins made exclusively in the cytosol?______________

E. Entrance into which organelle(s) requires that proteins unfold and snake through the membrane? _________________

F. How do organelles maintain their position in the cytosol? Be specific.

G. Give 2 advantages that eukaryotic cells gain by having organelles.

tealtoad562

1a.) What do the vast majority of proteins routed through the secretory pathway have in common?

A. They will be secreted.

B. The are post-translationally targeted to their final destination.

C. They are initially post-translationally inserted into the endoplasmic reticulum (ER) lumen.

D. They are co-translationally inserted into the ER lumen.

1b.) Which of the following accurately describes targeting proteins to the mitochondrial matrix or the nucleus?

A. Both mitochondrial matrix and nuclear proteins are co-translationally targeted to their destinations.

B. Both mitochondrial matrix and nuclear proteins are folded only after they reach their destinations.

C. Proteins targeted to the nucleus are completely folded in the cytoplasm. Proteins targeted to the mitochondrial matrix are unfolded while being inserted into the mitochondria and are refolded within the matrix.

D. Proteins targeted to the mitochondrial matrix are completed folded in the cytoplasm. Proteins targeted to the nucleus are unfolded while being inserted into the nucleus and are refolded within the nucleus.

E. None of these statements are correct.