Question

Enzyme Activity

1. Briefly explain the principle of spectrophotometry.

2. Which of the following statements is true about enzymes?(check all correct answers)

		__ enzymes act on (are specific for) one or just a fewsubstrates
		__ enzymes can be denatured (loose their tertiary/quaternarystructure and activity) by extreme conditions such as heat, high orlow pH,or presence of organic solvents
		__most enzymes are DNA
		__ enzymes are protein
		__enzymes perform best at specific conditions of temperature, pHand salt concentration 5. Which enzymes break the following macromolecules? Match the following with their correponding answers: ___ Nucleic acids A. proteases or proteinases such as pepsin ___ Cellulose B. none ___ Triglycerides C. lipases ___ Proteins D. amylases ___ Starch E. nucleases 6.Match the following enzymes totheir products. Answers ___ pepsin, trypsin and other digestive proteases A.Nucleotides ___ salivary and pancreatic amylases and other enzymes B. AminoAcids ___ lipases C. Monosaccharides ___ nucleases D. fatty acids and glycerol 7. Based on experiments, what is the effect of extremeconditions of pH and temperature (boiling tube) on enzymeactivity? 8. ATP stores energy in the form of___ ___ Chemical energy ___ Kinetic energy ___ Thermal energy ___ Activation energy 9. An allosteric site in anenzyme is____ __ the site where reactions occur __ the site that binds aregulator __ the site that binds thesubstrate __ the site that binds the product 10. Enzymes increase the rate of reactions by making thetransition state less stable. __ True __ False 11. Enzymes increase the rate of reactions by placing reactingparts of the molecule in favorable positions. __ True __ False 12. Which of the followingis a mechanism for regulation of enzymes? __ covalent modification __ feedback inhibition __ allosteric regulation __ all of the above 13. Which of the following represents a correct order of eventsin an enzyme catalytic cycle? 1.enzyme changes shape 2.substrate binds to active site 3.substrate is converted to products 4. products are released from activesite __ 1>2>3>4 __ 2>1>3>4 __ 3>2>1>4 __ 2>4>3>1