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2. Trypsin is a hydrophilic protease and therefore is unable to cross the plasma membrane and enter a cell. However, it is capable of digesting the hydrophilic portions of membrane proteins. As a result of these properties, researchers investigating membrane proteins have treated cells with trypsin then separated the proteins by SDS-PAGE to gain insight into the orientation of particular proteins within the membrane. Describe an experiment using this information and the results you would expect with the following types of membrane-associated proteins. (Assume the proteins are single polypeptides.) a. an integral membrane protein with the N-terminus exposed on the surface b. an integral membrane protein with the C-terminus exposed on the surface c. an integral membrane protein with three hydrophilic loops (only) exposed on the surface d. a membrane-associated protein facing the cytoplasm e. an imbedded membrane protein

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Jamar Ferry
Jamar FerryLv2
28 Sep 2019

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Related questions

9. Photosynthesis in plants requires the presence of the pigment ______________________, which is produced by _____________________________ within their cells.

10. Cytoskeleton is to animal cells as ______________________________ are to plant cells and bacteria.

11. Integral membrane proteins have both hydrophilic and ______________________regions that allow them to pass through the plasma membrane.

12. The prokaryotic world of bacteria have the most diverse types of _____________________________on their outer surfaces.

13. Some of a cell's __________________ are found on the surface of the rough endoplasmic reticulum.

14. The organelle that digests inorganic wastes, like H2O2, is known as the ____________________________.

15. The organelle that synthesizes proteins in eukaryotic cells is known as _____________________________.

16. A signaling molecule may bind to a protein on the surface of the plasma membrane. This protein is known as a/an ________________________.

17. The best analog to our ability to see, hear, smell and taste on the cell level comes in the form of protein receptors in the cell’s ________________________________________.

18. The supportive framework of a cell composed of microfilaments, intermediate filaments, and microtubules is known as the ____________________________________.

19. The organelle that contains enzymes for intracellular digestion and programmed cell death is known as the _____________________________.

20. Any solution that is more dilute than cytoplasm, which would cause a cell to swell with water is known as a/an __________________________________ solution.

________30. If you are investigating the inner structure of a new cell and see that it has a significantly larger amount of RER than other cells. You would conclude the cell does a great deal of____?
A. ATP synthesis

B. protein synthesis
C. DNA synthesis
D. active transport
E. polysaccharide hydrolysis

________31. If a cell comes in contact with a toxic substance such as H2O2, it could survive as long as it has a ___ to digest it.
A. DNA
B. peroxisome

C. mitochondria
D. phospholipids
E. intermediate filaments

________32. Some cells have structures to help them withstand osmotic pressure. For example,
A. Plant cells due to their walls
B. Animal cells due to the sodium/potassium pumps

C. Bacterial cell due to their swimming

D. Viruses due to their protein coats

________33. Evolution occurs only as a cell ____.

A. They undergo mitosis
B. Change in their gene pool
C. They move from one habitat to another

D. They begin to eat different foods

________34. Ribosomes are produced in the

A. nucleoli.
B. chromatin.
C. nuclear envelopes.

D. Golgi complex. E. cisternae.

________35. Algae are often green, but there are red and brown species also. What accounts for this difference in color?
A. The chlorophyll of chloroplasts
B. The pigments in other plastids

C. The presence of a mitochondria
D. The accumulation of cellular wastes

________36. The chloroplast and other like prokaryotes have which of the following that helps with photosynthesis?
A. They live in water based environments
B. They have chloroplasts in their cytoplasm

C. Change color in the dark vs. the sun
D. They have photoreceptor proteins in their membranes or mesosomes.

________37. Located in the membrane, we see a set of proteins called receptors that only connect with or bind to specific substances. This is called __.
A. ligand specificity.
B. hormone selectivity.

C. cell tonicity.
D. water saturation. E. signal resolution.

________38. Of the following statements, we could say that ___ is false.
A. All organisms are composed of cells and cell products.
B. An organism's structure and all of its functions are due to the activities of cells.
C. The cellular organelles are the simplest structural and functional unit of life.
D. Cells come from preexisting cells.
E. Cells are made from nonliving components including the nucleus, cell membrane and cytoplasm.

________39. If it is really true that our cells are the smallest anatomical unit that meets all of the properties of life, then which of the following could imply otherwise?
A. vacuoles
B. centrioles

C. Golgi apparatus
D. mitochondria
E. endoplasmic reticulum

________40. Oxygen is an absolute need for animal and plant cells alike due to the ___.

A. RER
B. Mitochondria
C. Lysosomes

D. DNA

________41. In a plasma membrane, we expect to see the carb/protein structures to extend into the ___, but the secondary messengers to move within the __.
A. extracellular fluid; cytosol
B. cytosol; extracellular fluid

C. intracellular fluid; cytosol

D. cytosol; intracellular fluid

E. extracellular fluid; plasma

________42. Bacteria are found in all of the following habitats:

A. Caves
B. Oceans
C. Animals

D. Everywhere on Earth

________43. Two solutions are separated by a selectively permeable membrane. Solution A has a higher concentration of an impermeable solute compared to solution B. Which of the following do you expect would happen?
A. solute will move from solution A to solution B

B. solute will move from solution B to solution A

C. water will more from solution A to solution B

D. water will move from solution B to solution A E. no movement of solute or water will occur

________44. In the kidney, we notice that almost all of the cells involved in the production of urine have high concentrations of the Na+/K+ pump. Which typically works to push Na+ ___ its concentration gradient by using ___.
A. up; active transport

B. up; facilitated transport
C. up; cotransport
D. down; active transport
E. down; facilitated transport

________45. In the charts of a patient recently admitted to the hospital noted that he was taking a medication called lithium and high doses of calcium supplements. Given this, we would expect the patient’s liver to have a higher than normal amount of __ to purify out the lithium and calcium.

A. lysosomes.
B. centrosomes.
C. mitochondria.
D. peroxisomes.
E. rough endoplasmic reticulum.

________46. We have long observed that our neural cells in the brain cannot replicate or replace themselves due to the lack of ____.
A. produce ATP.
B. divide.

C. produce enzymes.
D. secrete material across their plasma membrane E. conduct electrical signals.

________47. An organism’s structure and function is ultimately due to the structure and function of it’s __.
A. Organs
B. Tissues

C. Cells

D. Nuclei

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