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DNA sequencing & Bioinformatics

- Why are primers included in the DNA sequencing reaction?

- How does the base sequence lead to an identification of a species?

- Why might you not get a species identified?

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Jean Keeling
Jean KeelingLv2
28 Sep 2019

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Related questions

I need help with two questions regarding DNA sequencing...


1) Why might you find that your DNA isolation might havemultiple "perfect" matches (1.000) for more than one species? Giventhat 16S genes are used almost universally to discrimanate betweenspecies, How can you explain this result?


2) Why might you obtain a sequence trace that shows very strongsignal (tall, well-isolated peaks) but that nontheless has bothmajor and minor peaks at most nuceotide positions. What does thisresult indicate? Why might you fail to get any sequence matchscoring above 0.7000?



Identification of Unknown Bacteria by sequencing rDNA. (Microbiology)

Where, in the target DNA, do primers bind during PCR (conserved vs. variable)? How many variable regions are present in rDNA?

How many variable regions are present in rDNA?

Why did you run agarose gel electrophoresis?

How will you find out that the amplified DNA fragment is our desired target region?

Thanks

PLEASE ANSWER ALL PARTS FULLY!

How was liquid hybridization used to compare species relatedness? What is the purpose/significance of Driver versus Tracer DNA? What is Subtractive Hybridization, how/why would you use this technique?

Compare Maxam-Gilbert and Sanger DNA sequencing methods. What are the more descriptive names for these techniques? How are the techniques similar? different? What reagents are used in each reaction? How are the results read? Do you get the sequence of the molecule you started with or the complementary molecule? How does fluorescent cycle sequencing differ from the original Sanger Method?

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