Do I use the banana or potato? Please read! How can I create anexperiment testing the effects of low pH on enzyme activity?
Hello! I'm having troubles getting started with my scienceexperiment. The instructions are: Design an experiment in which youwill test the effect of an acidic fluid on enzymatic activity. IDthe enzyme, the substrate, the acidic fluid used as treatment, thecontrol treatment and the method of measuring enzyme activity, aswell as explain your experimental protocol. You must alsothoroughly explain how the acidic fluid impacted enzyme activitybased on the results from your own experiment as well as knowledgeof enzymes and pH.
Here is what I have so far:
I have five test tubes, and Iâm supposed to be testing the effectsof low pH on enzyme activity. Iâve chosen Amylase/banana as myenzyme and starch/potato as my substrate. (I also have left overstarch liquid and IKI.) I was going to test the different pHs byadding water (7), draino (14), soda (4), banana (5) and soda (4) to1 cubic centimeter of a potato in each tube. I guess Iâm just notsure if Iâm doing it right. The independent variable influences thechange in my dependent variable. Is my dependent variable thepotato? My independent variable would be the water, draino, soda,banana and soda. What would my control be? Thank you for your help.Am I supposed to put 1 cubic centimeter of potato in each test tubeand then only put the banana in one test tubes with the potato orget rid of the potato and only use the banana in each testtube?
Enzyme:Amylase/banana
Substrate: Starch/Potato
Control: ?
Independent Variable: H2O, Draino, Soda, Banana
Dependent Variable: Potato
Hypothesis: High levels of pH will slow downreactions and low levels of pH will speed them up.
Null Hypothesis: High levels of pH will speed upreactions and low levels of pH will slow them down.
Do I use the banana or potato? Please read! How can I create anexperiment testing the effects of low pH on enzyme activity?
Hello! I'm having troubles getting started with my scienceexperiment. The instructions are: Design an experiment in which youwill test the effect of an acidic fluid on enzymatic activity. IDthe enzyme, the substrate, the acidic fluid used as treatment, thecontrol treatment and the method of measuring enzyme activity, aswell as explain your experimental protocol. You must alsothoroughly explain how the acidic fluid impacted enzyme activitybased on the results from your own experiment as well as knowledgeof enzymes and pH.
Here is what I have so far:
I have five test tubes, and Iâm supposed to be testing the effectsof low pH on enzyme activity. Iâve chosen Amylase/banana as myenzyme and starch/potato as my substrate. (I also have left overstarch liquid and IKI.) I was going to test the different pHs byadding water (7), draino (14), soda (4), banana (5) and soda (4) to1 cubic centimeter of a potato in each tube. I guess Iâm just notsure if Iâm doing it right. The independent variable influences thechange in my dependent variable. Is my dependent variable thepotato? My independent variable would be the water, draino, soda,banana and soda. What would my control be? Thank you for your help.Am I supposed to put 1 cubic centimeter of potato in each test tubeand then only put the banana in one test tubes with the potato orget rid of the potato and only use the banana in each testtube?
Enzyme:Amylase/banana
Substrate: Starch/Potato
Control: ?
Independent Variable: H2O, Draino, Soda, Banana
Dependent Variable: Potato
Hypothesis: High levels of pH will slow downreactions and low levels of pH will speed them up.
Null Hypothesis: High levels of pH will speed upreactions and low levels of pH will slow them down.
Related textbook solutions
Related questions
Enzymes Lab Report
- Enzyme Structure and Function
- Define the following:
- enzyme
- substrate
- Explain how enzymes function.
- Go to Lab, Section I, Exercise 1 to participate in an activitythat demonstrates the importance of enzymes. Record the colorintensity of the solution in each test tube at five and tenminutes.
| Time (min) | Tube S1 Potato Extract + Catechol | Tube S2 Potato Extract + Water | Tube S3 Catechol + Water |
0 | |||
5 | |||
10 |
- How can benzoquinone be detected?
- Why were potatoes used in the experiment?
- Enzyme Specificity
- Based on Section II, Exercise 2, as determined by colorintensity, to what extent does catechol oxidase react withhydroquinone?
- What was the role of hydroquinone in the experiment?
- Temperature and Enzyme Activity
- Based on Lab, Section III, Exercise 3 record the relative colorintensity at five-minute intervals to determine the temperaturerange for catechol oxidase.
| Time (min) | Tube 1 4oC | Tube 2 23oC | Tube 3 40oC | Tube 4 60oC | Tube 5 80oC | Tube 6 100oC |
0 | ||||||
5 | ||||||
10 |
- Construct a bar graph of the data for the ten-minute readings.Label axes and provide units. Sign, date and prepare an image ofyour graph and include it with this lab report.
- What is the optimal temperature range for catechol oxidaseactivity?
- pH and Enzyme Activity
- Within what pH range will catechol oxidase catalyze catecholand oxygen to form benzoquinone?
- Define pH.
- What is the role of catechol in the experiment?
- Which test tube contains the most acidic buffer solution?
- Cofactors and Enzyme Activity
- What is a cofactor?
- What cofactor is necessary for catechol oxidase activity?
Summary Questions
- What was the role of catechol oxidase in the experiments?
- What was the purpose of developing a color intensityscale?
- Identify three factors that influence the rate of enzymaticreactions.
- Examine the data in Exercise 3 and suggest a hypothesisexplaining why people with a fever feel tired and listless.
- People who have been suddenly submerged in cold water andremained for long periods of time have survived. Examine the datain Exercise 3 and explain how this is possible.
- How does pH range affect the function and structure of catecholoxidase?
- The stomach maintains a pH of approximately 3 to 5. Do allenzymes function at this pH level? Examine the data in Exercise 4and determine if catechol oxidase would function in the stomach.
- What is enzyme specificity? Give an example.
