In the tomato, red fruit (R) is dominant over yellow fruit (r), and yellow flowers (F) are dominant over white flowers (f). A cross was made between true-breeding plants with red fruit and yellow flowers, and plants with yellow fruit and white flowers. All F1 offspring are heterozygotes for both traits. The F1 female plants were then test crossed to males with yellow fruit and white flowers (homozygous recessive for both traits). The following results were obtained:
333 red fruit, yellow flowers
64 red fruit, white flowers
58 yellow fruit, yellow flowers
345 yellow fruit, white flowers
Total of 800
1. Conduct a Chi square analysis to see if the two genes are linked ( Check a solved problem S1, page 148 in the first edition text book, and page 150 in the second edition problem #1 for an idea)â¦follow the same steps)
a. Propose a hypothesis to be tested
b. Find the expected (predicted) number of offspring ( Hint what is the ratio of test cross results?)
c. Calculate and Interpret the Chi âsquare result
d. If the two genes (fruit color and flower color) are linked, calculate the map distance between the two genes.
2. Set a sample test cross, Explain the rationale behind a testcross, why is it necessary for one of the parents to be homozygous recessive for the genes of interest?
In the tomato, red fruit (R) is dominant over yellow fruit (r), and yellow flowers (F) are dominant over white flowers (f). A cross was made between true-breeding plants with red fruit and yellow flowers, and plants with yellow fruit and white flowers. All F1 offspring are heterozygotes for both traits. The F1 female plants were then test crossed to males with yellow fruit and white flowers (homozygous recessive for both traits). The following results were obtained:
333 red fruit, yellow flowers
64 red fruit, white flowers
58 yellow fruit, yellow flowers
345 yellow fruit, white flowers
Total of 800
1. Conduct a Chi square analysis to see if the two genes are linked ( Check a solved problem S1, page 148 in the first edition text book, and page 150 in the second edition problem #1 for an idea)â¦follow the same steps)
a. Propose a hypothesis to be tested
b. Find the expected (predicted) number of offspring ( Hint what is the ratio of test cross results?)
c. Calculate and Interpret the Chi âsquare result
d. If the two genes (fruit color and flower color) are linked, calculate the map distance between the two genes.
2. Set a sample test cross, Explain the rationale behind a testcross, why is it necessary for one of the parents to be homozygous recessive for the genes of interest?