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13 Aug 2018

Microbiologists describe the processes of transcription and translation as âcoupledâ in bacterial cells. This term indicates that a bacterial mRNA can be undergoing transcription at the same time that it also is undergoing translation.

a. How is coupling of transcription and translation possible in bacteria?

b. Is coupling of transcription and translation possible in single-celled eukaryotes such as yeast? Why or why not?

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Reid WolffLv2

14 Aug 2018

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Related questions

22. What is probably the single most important distinction between bacteria and eukaryotes?

a. transcription

b. the separation of the cell's genetic material from the surrounding cytoplasm

c. the existence of ribosomes

d. RNA processing in bacteria

e. antibiotics

23. Which one does NOT characterize an open reading frame (ORF)

a. Uses six reading frames during translation

b. Contains a start codon

c. Contains a stop codon

d. Its length is in multiples of 3 nucleotides

e. It should have the number of codons for a protein or on average ~250 codons in length.

24. Nested intronic genes lie within the non-coding intronic region of a larger gene, and occur in the human genome.

a. True

b. False

25. How would you characterize this statement: EF-Tu will hydrolyze GTP only if the codon:anticodon & tRNA:amino acid matchings are correct

a. Transcription; initiation steps

b. Transcription; termination steps

c. Translation: initiation steps

d. Tanslation: elongation steps

e. Spliceosome

26. Regarding the transcription unit architecture of the Escherichia coli genome, which one is FALSE?

a. Each of the 4,661 transcription units comprised an average of 1.1 modular units.

b. 3,010 transcription units (~65%) are monocistronic.

c. 1,652 transcription units (35%) contain more than one ORF (polycistronic)

d. The largest transcription unit is equivalent to 16 ORFs with numerous introns.

e. 398 transcription units (~9%) are nested within each other in opposite transcriptional orientation

27. Regarding RNA interference (RNAi), which one is FALSE?

a. It is a common and conserved mechanism in eukaryotic cells.

b. Originally discovered in bacteria and plant viruses.

c. A form of primitive immunity to protect the genome.

d. Pre microRNA (miRNA) genes have been found in introns.

e. Recent research shows that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

28. Aminoacyl-tRNA synthetases

a. Recognize the 3âUTR

b. The genes for these enzymes fall into the family of inducible genes

c. The enzymes are imported into the nucleus

d. Covalently attaches an amino acid to the appropriate tRNA. This takes place in the cytoplasm.

e. Forms the peptide bond during protein synthesis

29. What is the process by which complementary RNAs are made from a DNA template?

a. translation

b. transcription

c. protein synthesis

d. transduction

e. transliteration

cyanbee225

A. To make an mRNA transcript from a gene, the RNA polymerase needs to 'know' where to start and to stop. What are the signals and proteins involved in initiating and terminating RNA polymerase activity in E. coli?

B. Alternative splicing is a key feature of the eukaryotic transcription-translation process.

i) In eukaryotes, alternative splicing of pre-mRNAs can result in the production of many structurally distinct proteins from information encoded in a single gene. Explain.

ii) Do lower eukaryotes (such as yeast, Saccharomyces cerevisiae) have the same opportunity to use alternative splicing to generate a vast array of proteins from a single gene? Explain.

iii) What other splicing events can lead to additional diversity in the repertoire of proteins that could be encoded by a single gene? Hint: think of the N and C terminal ends. What about a post-translational mechanism. Name one.

If the bold term in each statement is correct hen write the letter C next to it. if the term is incorrect then replace it with the correct term.

The nucleotide bases found only in RNA and not in DNA is thymine.

The class of RNA molecules that carries the amino acids that are added to the growing polypeptide chain is tRNA.

During translation immediately after the first and second tRNA molecules become attached side by side on the mRNA., a hydrogen bond is formed between adjacent amino acids.

In the genetic code, 20 bases on the DNA code for a single amino acid.

The major lipid involved is transcription is RNA polymerase.

The one strand of DNA used as a template in the making of mRNA during transcription, is the template strand.