A. To make an mRNA transcript from a gene, the RNA polymerase needs to 'know' where to start and to stop. What are the signals and proteins involved in initiating and terminating RNA polymerase activity in E. coli?
B. Alternative splicing is a key feature of the eukaryotic transcription-translation process.
i) In eukaryotes, alternative splicing of pre-mRNAs can result in the production of many structurally distinct proteins from information encoded in a single gene. Explain.
ii) Do lower eukaryotes (such as yeast, Saccharomyces cerevisiae) have the same opportunity to use alternative splicing to generate a vast array of proteins from a single gene? Explain.
iii) What other splicing events can lead to additional diversity in the repertoire of proteins that could be encoded by a single gene? Hint: think of the N and C terminal ends. What about a post-translational mechanism. Name one.
A. To make an mRNA transcript from a gene, the RNA polymerase needs to 'know' where to start and to stop. What are the signals and proteins involved in initiating and terminating RNA polymerase activity in E. coli?
B. Alternative splicing is a key feature of the eukaryotic transcription-translation process.
i) In eukaryotes, alternative splicing of pre-mRNAs can result in the production of many structurally distinct proteins from information encoded in a single gene. Explain.
ii) Do lower eukaryotes (such as yeast, Saccharomyces cerevisiae) have the same opportunity to use alternative splicing to generate a vast array of proteins from a single gene? Explain.
iii) What other splicing events can lead to additional diversity in the repertoire of proteins that could be encoded by a single gene? Hint: think of the N and C terminal ends. What about a post-translational mechanism. Name one.
