Do these two different individuals share any bands of the same length? Why might this be the case?

Do these two different individuals share any bands of the same length? Why might this be the case?

Yes. Each band is represented in a certain percentage of the population, so even unrelated individuals have a few bands in common.

No. No two individuals have bands of the same length on a DNA profile.

Yes, but only because one DNA sample must have been contaminated with the other sample.

Yes. The two individuals are siblings.

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Elin HesselLv2

13 Jun 2019

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Related questions

You perform electrophoresis on the product of a PCR and see that instead of the 300-baseband you expected that there are five different bands.

What controls the specific sequence to which a PCR primer binds? complementary sequence

What are two factors that contribute to the annealing temperature of the primer? primer length and the GC content (What does GC stand for)

What part of your thermal cycle protocol could you change, and how would you change it, to try and get rid of the extra bands?

Thermal cycle protocol could you change -

Change it - temperature should be increased to try to get rid of the extra bands.

d. If, after performing electrophoresis, there were no bands on the gel, what part of your thermal cycle protocol would you change, and how would you change it?

Thermal cycle protocol could you change -

Change it - temperature should be decreased to allow binding of the primer.

If you are using a DNA sequence from a related species to design primers for your species of interest, why is it better to design your primers to complement an exon?

Why is it better to use a 25-base primer than a 10-base primer? Why do you have to have a lower annealing temperature for a 10-base primer?

Thank I appreciate it if you could help with these three questions. Ran out of questions to ask

Thank you again.

Plaease help me to answer these question of gel electrophresis class.

Q1: What is electrophresis and why do you need to run a DNA ladder together with your sample ?

Q2 In the well where you ran pUC19 (plasmid sample with known concentration for positive contorol) , you will probably see more than one band. The purification with the silica column allows for the elimination of chromosomal contaminants, so what types of DNA molecules might be present in the different bands ( In order to ansewer this question, you need to find out how plasmids repilicate and what the properities of supercoiled plasmids are)

I can ansewer to Q1 by myself (I also asked for Q1, because I want to check my own ansewer) , but to Q2, I can't.

Answered most of the questions. Can you tell me if my answers are correct? Last question of the month. Thank you. I appreciate it.

4. You perform electrophoresis on the product of a PCR and see that instead of the 300-base band you expected that there are five different bands.

What controls the specific sequence to which a PCR primer binds? Complementary sequence and annealing temperature control the specific sequence to which a PCR primer binds.

What are two factors that contribute to the annealing temperature of the primer? Two factors that contribute to the annealing temperature of the primer is primer length and the Guanine and Cytosine content.

What part of your thermal cycle protocol could you change, and how would you change it, to try and get rid of the extra bands?

To get rid of the extra band, we have to increase the annealing temperature.

If, after performing electrophoresis, there were no bands on the gel, what part of your thermal cycle protocol would you change, and how would you change it?

After performing electrophoresis, if there were no bands on the gel then we have to decrease the annealing temperature of the primer

5. If you are using a DNA sequence from a related species to design primers for your species of interest, why is it better to design your primers to complement an exon?

In related species there are similarities but their genetic makeup will not be the same. It is better to design your primers to complement an exon because ??

6. Why is it better to use a 25-base primer than a 10-base primer? Why do you have to have a lower annealing temperature for a 10-base primer?

It is better to use a 25-base primer than a 10-base primer because a 10-base primer is not long enough and may pair with a wrong portion of DNA, which would give wrong results. You have a lower annealing temperature for a 10-base primer because annealing temperature increases when the number of base pairs increase. If the temperature is too high the primer might not bind.

Identify the three steps in PCR and tell the function of each step.

Denaturation - when the double-stranded DNA is heated to 92-95â for about one minute, to separate it into two single strands.

Annealing - temperature of the reaction is lowered to a temperature that is between 45â and 65â to allow the DNA primers to attach to the template DNA.

Extension - temperature of the reaction is raised to a temperature between 45â and 65â and the new strand of DNA is made by adding nucleotides to the ends of the primers.